《植物生理学报》 2009, 45(8): 791-793

过量表达腺苷甲硫氨酸合成酶基因能提高转基因烟草中多聚胺的生物合成

戚元成^1,2, 马雷¹, 王菲菲², 刘卫群^1,2,*

河南农业大学1 烟草学院, 2 生命科学学院, 郑州450002

通信作者：刘卫群；E-mail: liuweiqun2004@126.com；Tel: 0371-63555153

摘　要：

以土壤农杆菌介导的转化方法将盐地碱蓬的腺苷甲硫氨酸合成酶 cDNA (SsSAMS2)转化到烟草 K326 中。PCR 分析 的结果表明, SsSAMS2 整合进 K326 的基因组内。共筛选到 8 个转基因纯合品系, 分析其中 3 个品系(ST8-9、ST14-2、ST3-5)基因表达和多聚胺含量的结果表明, SsSAMS2可在转基因烟草中表达, 转基因烟草中的多聚胺含量明显高于野生型烟草。 这些结果表明, 腺苷甲硫氨酸合成酶基因已在转基因烟草中过量表达并导致多聚胺含量提高。

关键词：腺苷甲硫氨酸合成酶; 多聚胺; 转基因烟草

收稿：2009-05-03   修定：2009-06-23

资助：国家“863” 计划(2002AA629080)和国家烟草专卖局重点项目(110200001011A)。

Overexpression of S-adenosylmethionine Synthetase Promote Polyamine Biosynthesis in Transgenic Tobacco

QI Yuan-Cheng^1,2, MA Lei¹, WANG Fei-Fei², LIU Wei-Qun^1,2,*

¹College of Tobacco Science, ²College of Life Science, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: LIU Wei-Qun; E-mail: liuweiqun2004@126.com; Tel: 0371-63555153

Abstract:

The full-length S-adenosylmethionine synthetase (SsSAMS2) of Suadea salsa was introduced into tobacco (Nicotiana tabacum L. K326) by Agrobacterium tumefaciens-mediated transformation. The gene integration in tobacco was confirmed by PCR. Eight homozygous transgenic lines (ST lines) were screened. The result of SsSAMS2 expression and polyamines (PAs) content analysis of three lines (ST8-9, ST14-2, ST3-5) showed that SsSAMS2 expressed in transgenic tobacco, and transgenic tobacco accumulated more soluble total PAs content than wild-type plants. These results indicated that the promotion of soluble total PAs content resulted from the SsSAMS2 overexpression in transgenic plants.

Key words: S-adenosylmethionine synthetase; polyamine; transgenic tobacco